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Mike and Hanna,
When you're comparing the image that's been saved multiple times vs. the original image, enlarge the images to 300+ percent and look at one small area of the images. A drop of water perhaps. You should see a difference. I'm not surprised that you don't see a difference in images saved multiple times as a maximum quality jpg, and then posted on the forum, as they're only displaying 72 dpi.
Just to prove to yourself that jpg is a lossy format, try saving the same image, 20 times, at the lowest quality jpg setting, and there should be a very noticeable difference (without having to magnify the image to see it). Very nice closeups, by the way.
Holly
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